Interrogating Transcriptional Regulatory Sequences in Tol2-Mediated Xenopus Transgenics
نویسندگان
چکیده
منابع مشابه
Interrogating Transcriptional Regulatory Sequences in Tol2-Mediated Xenopus Transgenics
Identifying gene regulatory elements and their target genes in vertebrates remains a significant challenge. It is now recognized that transcriptional regulatory sequences are critical in orchestrating dynamic controls of tissue-specific gene expression during vertebrate development and in adult tissues, and that these elements can be positioned at great distances in relation to the promoters of...
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Xenopus is a powerful model for studying a diverse array of biological processes. However, despite multiple methods for transgenesis, relatively few transgenic reporter lines are available and commonly used. Previous work has demonstrated that transposon based strategies are effective for generating transgenic lines in both invertebrate and vertebrate systems. Here we show that the Tol2 transpo...
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The Nile tilapia (Oreochromis niloticus) is an important species in aquaculture and an excellent model system for laboratory studies. Functional genetic analysis using this species has been difficult because existing methods for producing transgenics are inefficient. Here we show that the Tol2 transposon system can be used to create transgenic tilapia with high efficiency. We constructed a line...
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Development is controlled by a complex series of events requiring sequential gene activation. Understanding the logic of gene networks during development is necessary for a complete understanding of how genes contribute to phenotype. Pioneering work initiated in the sea urchin and Drosophila has demonstrated that reasonable transcriptional regulatory network diagrams representing early developm...
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The generation of zebrafish transgenic lines that express specific fluorophores in a cell- or tissue-specific manner is an important technique that takes full advantage of the optical clarity of the embryo. Identifying promoter fragments that faithfully recapitulate endogenous expression patterns and levels is often difficult and using large genomic DNA fragments, such as bacterial artificial c...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2013
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0068548